Protein Mass Spectrometry Sample Prep
Waters Corp WATERS CORPORATION GlycoWorks RapiFluor-MS N-Glycan Eco Starter Kit, 96 Samples
This kit is designed to provide scientists with all the necessary materials to successfully perform fast N-glycan deglycosylation, rapid fluorescent labeling, and glycan extraction with minimal preparation time. It uses optimized deglycosylation conditions and reagents, a novel rapid label called RapiFluor-MS for sensitive fluorescence and mass detection, and solid-phase extraction (SPE) for sample clean-up prior to LC analyses.
- Optimized deglycosylation conditions and reagents for fast release.
- Features RapiFluor-MS, a novel rapid label for sensitive fluorescence and mass detection.
- Sample clean-up via solid-phase extraction (SPE) with the HILIC μElution™ Plate.
- Streamlined protocol for fast sample preparation, minimizing handling and loss.
- Each reaction is optimized for the release, labeling, and extraction of N-glycans from 15 to 40 μg of glycoprotein.
Waters Corp GlycoWorks RapiFluo-MS N-Glycan Eco Starter Kit, 96 Samples, UPLC Glycan BEH Column
The GlycoWorks RapiFluo-MS N-Glycan Eco Starter Kit offers a fast, sensitive method for released N-glycan analysis. It simplifies sample preparation to three steps in 30 minutes. Using RapiFluor-MS (RFMS) technology, it provides increased fluorescence and superior mass spectral response for characterization and monitoring with a single label. The “Eco” design replaces DMF with DMSO as a safer reagent solvent.
- Reduces N-glycan sample preparation to 3 easy steps in as little as 30 minutes.
- Provides increased fluorescence quantification and superior mass spectral response with RapiFluor-MS technology.
- Enables versatile labeling, using a single label for both characterization and routine monitoring.
- Replaces DMF with DMSO as a greener and safer reagent solvent, making it eco-friendly.
- Includes all necessary components for N-glycan analysis.
Waters Corp Waters GlycoWorks Kit, RapiFluor-MS N-Glycan, Automation, ACQUITY Glycan Column
The Waters GlycoWorks RapiFluor-MS N-Glycan Eco Script Parameters Starter Kit with ACQUITY Premier Glycan BEH Amide Column — Automation is designed for fast enzymatic release and rapid labeling of N-glycans. It facilitates high-throughput glycoprotein sample preparation on automated platforms, processing 48 or 96 samples. The kit includes optimized deglycosylation conditions, RapiFluor-MS for sensitive fluorescence and enhanced mass detection, and a GlycoWorks HILIC μElution Plate for sample cleanup. Full automation for 96 samples can be completed in under 3 hours.
- Fast enzymatic release and rapid labeling of N-glycans.
- Scaled to process 48 or 96 samples for high-throughput automation.
- Contains RapiFluor-MS for sensitive fluorescence and enhanced mass detection.
- Includes solid-phase extraction (SPE) for sample cleanup.
Bioss Ureaplasma sp.
Ureaplasma sp Primary Antibody Unconjugated Host Species Mouse Target Species Clonality Monoclonal Applications WB ELISA Concentration 100 g/ml Size 100 ug Storage Buffer PBS (pH 7 2) with 0 1% azide
Waters Corp GlycoWorks RapiFluor-MS N-Glycan Kit, Automation, 96 Analysis, Sample Preparation Workflow
The GlycoWorks RapiFluor-MS N-Glycan Kit – Automation is designed for high-throughput glycoprotein sample preparation for N-glycan analysis with minimal user intervention. This protocol has been validated using monoclonal antibodies and has also been tested for a wide range of other N-linked glycoproteins. All reagents in the kit are scaled to residual volumes needed for many automated instrumentation, allowing for versatility when choosing your platform. The kit is in a 2 x 48 format.
- Designed for automated platforms, enabling high-throughput glycoprotein sample preparation.
- Minimizes user intervention.
- Protocol validated with monoclonal antibodies and tested for a wide range of other N-linked glycoproteins.
- Reagents scaled to residual volumes for versatility across automated instrumentation.
- Kit format: 2 x 48.
Waters Corp N-Glycan Eco Script Starter Kit, GlycoWorks RapiFluor-MS, Automation, 2 x 48 Format
The GlycoWorks RapiFluor-MS N-Glycan Eco Script Starter Kit – Automation is engineered for high-throughput glycoprotein sample preparation on automated platforms, requiring minimal user involvement for N-glycan analysis.
- Includes a CD and workstation requirements document containing the script developed during verification testing for automated systems.
- Contains an RFMS labeled standard, column, and mobile phase concentrate recommended for use with the RapiFluor-MS library.
Waters Corp ProteinWorks eXpress Digest Kit, LC-MS Protein Quantification, 96 Analysis
The ProteinWorks eXpress Digest Start-up Kit is designed to simplify and accelerate the direct digestion of immunopurified plasma & serum samples for LC-MS protein quantification using the surrogate peptide approach.
- Simplifies and accelerates direct digestion for LC-MS protein quantification of immunopurified plasma & serum samples.
- Provides a standardized, proven route for fast, accurate, and precise protein quantification in bioanalysis.
- Includes pre-measured, lot-traceable chemicals optimized for dependable LC-MS protein quantification.
- Enables rapid quantification, such as Infliximab down to 10 ng/mL.
- Achieves single-digit intra-kit and inter-kit reproducibility across multiple monoclonal antibodies.
Waters Corp GlycoWorks Rapi-Fluor-MS N-Glycan Eco Basic Kit, 24 samples
This GlycoWorks RapiFluor-MS N-Glycan Eco Kit streamlines N-glycan analysis with three easy, 30-minute steps. It uses RapiFluor-MS for enhanced fluorescence quantification and superior mass spectral response, and replaces DMF with a greener, safer DMSO solvent. The kit processes 24 samples.
- Streamlined N-glycan analysis with three easy, 30-minute steps.
- Enhanced fluorescence and mass spectral response with RapiFluor-MS.
- Eco-friendly kit replaces DMF with DMSO and processes 24 samples.
Waters Corp GlycoWorks RapiFluor-MS N-Glycan Starter Kit, 24 Samples, ACQUITY Premier UPLC Glycan BEH Column
This kit provides everything necessary for enzymatic deglycosylation, RapiFluor-MS labeling, SPE clean-up, and UPLC analysis, including a UPLC column and mobile phase concentrate. It offers a streamlined, fast, and highly sensitive method for analyzing released N-glycans.
- Three easy steps in as little as 30 minutes, reducing complicated sample preparation.
- RapiFluor-MS provides increased fluorescence quantification and supreme mass spectral response.
- Uses a single label for characterization to routine monitoring.
- Processes 24 samples in a 3×8 format.
- Includes a labeling module for the solubilization and labeling of released glycosylamine.
- Features a UPLC Glycan BEH Amide column, 1.7 μm, 2.1 × 150 mm.
Waters Corp N-Glycan Kit, Eco Basic, 96 Samples
This GlycoWorks RapiFluor-MS N-Glycan Eco Kit simplifies and accelerates glycan analysis sample preparation, reducing it to 30 minutes in three easy steps. Utilizing RapiFluor-MS (RFMS) for enhanced fluorescence quantification and superior mass spectral response, it allows a single label for characterization and routine monitoring. The “Eco” version uses greener DMSO as a reagent solvent, replacing DMF.
- Reduces sample preparation to 30 minutes in 3 easy steps.
- Enhanced detection provides increased fluorescence quantification and supreme mass spectral response.
- Single labeling technology can be used for both glycan characterization and routine monitoring.
- Eco-friendly as it replaces DMF with DMSO as a greener and safer solvent.
- Includes deglycosylation, eco labeling, and eco clean-up modules.
TA Instruments Tzero Powder Sample Preparation Kit, Designed for Powdered Samples, Provides Non Skid Base, Stainless Steel Tools and Optimized Loading for Reliable DSC Results
Waters TA Instruments Tzero Powder Sample Preparation Kit is designed for users working with powdered samples and Tzero pans. It includes a non skid base and three part stainless steel tool set to simplify loading. The design optimizes sample placement, prevents contamination, and ensures reliable, repeatable DSC results with powdered materials.
Enzo Life Sciences FLUOR DE LYS-Succinyl Green (50µl)
The FLUOR DE LYS-Succinyl Green, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5. The assay procedure has two steps. First, the FLUOR DE LYS-Succinyl Green, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS Developer (BML-KI105) produces a free rhodamine-based fluorophore which can be monitored with standard fluorometers (Ex. 470-500nm / Em. 520-550nm). . Formulation: Solution in DMSO. Purity: ≥95% (HPLC). Long Term Storage: -80°C.